Coumarin-like fluorescent molecular rotors for bioactive polymers probing.
Identifieur interne : 001643 ( Main/Exploration ); précédent : 001642; suivant : 001644Coumarin-like fluorescent molecular rotors for bioactive polymers probing.
Auteurs : P. Even [France] ; F. Chaubet ; D. Letourneur ; M L Viriot ; M C CarréSource :
- Biorheology [ 0006-355X ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- cytologie : Endothélium.
- pharmacologie : Polyosides.
- Colorants fluorescents, Coumarines, Division cellulaire, Endothélium, Humains, Lignée cellulaire, Microscopie de fluorescence, Polymères, Polyosides, Sondes moléculaires.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Coumarins, Polymers, Polysaccharides.
- cytology : Endothelium.
- drug effects : Cell Division, Endothelium.
- chemical , pharmacology : Polysaccharides.
- Cell Line, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Molecular Probes.
Abstract
Polysaccharides are interesting and often essential macromolecules but are difficult to analyse due to their lack of convenient chromophores. We propose an efficient labelling procedure for polysaccharides such as functionalized dextrans with coumarin derivatives: the fluorescent tracers present inter alia properties of emission of fluorescence dependent on the molecular environment (polarity, viscosity, temperature, pH, etc.). Hence, with in mind the understanding of cell-polysaccharide interactions, the labelled polymers were studied by in vitro tests on a line of endothelial cells sensitive to the proliferative effect of these dextran polysaccharides. Using 3D fluorescence microscopy, the fixation and internalization of fluorescent functionalized dextrans were observed in endothelial cells.
PubMed: 12454414
Affiliations:
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Le document en format XML
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Chaubet</name></author><author><name sortKey="Letourneur, D" sort="Letourneur, D" uniqKey="Letourneur D" first="D" last="Letourneur">D. Letourneur</name></author><author><name sortKey="Viriot, M L" sort="Viriot, M L" uniqKey="Viriot M" first="M L" last="Viriot">M L Viriot</name></author><author><name sortKey="Carre, M C" sort="Carre, M C" uniqKey="Carre M" first="M C" last="Carré">M C Carré</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12454414</idno><idno type="pmid">12454414</idno><idno type="wicri:Area/PubMed/Corpus">000210</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000210</idno><idno type="wicri:Area/PubMed/Curation">000210</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000210</idno><idno type="wicri:Area/PubMed/Checkpoint">000210</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000210</idno><idno type="wicri:Area/Ncbi/Merge">000025</idno><idno type="wicri:Area/Ncbi/Curation">000025</idno><idno type="wicri:Area/Ncbi/Checkpoint">000025</idno><idno type="wicri:doubleKey">0006-355X:2003:Even P:coumarin:like:fluorescent</idno><idno type="wicri:Area/Main/Merge">001892</idno><idno type="wicri:Area/Main/Curation">001643</idno><idno type="wicri:Area/Main/Exploration">001643</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Coumarin-like fluorescent molecular rotors for bioactive polymers probing.</title><author><name sortKey="Even, P" sort="Even, P" uniqKey="Even P" first="P" last="Even">P. Even</name><affiliation wicri:level="3"><nlm:affiliation>Département de Chimie Physique des Réactions, UMR 7630 CNRS-INPL, Groupe ENSIC, 1, rue Grandville, BP 451, 54000 Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département de Chimie Physique des Réactions, UMR 7630 CNRS-INPL, Groupe ENSIC, 1, rue Grandville, BP 451, 54000 Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName></affiliation></author><author><name sortKey="Chaubet, F" sort="Chaubet, F" uniqKey="Chaubet F" first="F" last="Chaubet">F. Chaubet</name></author><author><name sortKey="Letourneur, D" sort="Letourneur, D" uniqKey="Letourneur D" first="D" last="Letourneur">D. Letourneur</name></author><author><name sortKey="Viriot, M L" sort="Viriot, M L" uniqKey="Viriot M" first="M L" last="Viriot">M L Viriot</name></author><author><name sortKey="Carre, M C" sort="Carre, M C" uniqKey="Carre M" first="M C" last="Carré">M C Carré</name></author></analytic><series><title level="j">Biorheology</title><idno type="ISSN">0006-355X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Division (drug effects)</term><term>Cell Line</term><term>Coumarins (chemistry)</term><term>Endothelium (cytology)</term><term>Endothelium (drug effects)</term><term>Fluorescent Dyes</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Molecular Probes</term><term>Polymers (chemistry)</term><term>Polysaccharides (chemistry)</term><term>Polysaccharides (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Colorants fluorescents</term><term>Coumarines ()</term><term>Division cellulaire ()</term><term>Endothélium ()</term><term>Endothélium (cytologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Microscopie de fluorescence</term><term>Polymères ()</term><term>Polyosides ()</term><term>Polyosides (pharmacologie)</term><term>Sondes moléculaires</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Coumarins</term><term>Polymers</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Endothélium</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Endothelium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Division</term><term>Endothelium</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Polyosides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Polysaccharides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Fluorescent Dyes</term><term>Humans</term><term>Microscopy, Fluorescence</term><term>Molecular Probes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Colorants fluorescents</term><term>Coumarines</term><term>Division cellulaire</term><term>Endothélium</term><term>Humains</term><term>Lignée cellulaire</term><term>Microscopie de fluorescence</term><term>Polymères</term><term>Polyosides</term><term>Sondes moléculaires</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polysaccharides are interesting and often essential macromolecules but are difficult to analyse due to their lack of convenient chromophores. We propose an efficient labelling procedure for polysaccharides such as functionalized dextrans with coumarin derivatives: the fluorescent tracers present inter alia properties of emission of fluorescence dependent on the molecular environment (polarity, viscosity, temperature, pH, etc.). Hence, with in mind the understanding of cell-polysaccharide interactions, the labelled polymers were studied by in vitro tests on a line of endothelial cells sensitive to the proliferative effect of these dextran polysaccharides. Using 3D fluorescence microscopy, the fixation and internalization of fluorescent functionalized dextrans were observed in endothelial cells.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Nancy</li></settlement></list><tree><noCountry><name sortKey="Carre, M C" sort="Carre, M C" uniqKey="Carre M" first="M C" last="Carré">M C Carré</name><name sortKey="Chaubet, F" sort="Chaubet, F" uniqKey="Chaubet F" first="F" last="Chaubet">F. Chaubet</name><name sortKey="Letourneur, D" sort="Letourneur, D" uniqKey="Letourneur D" first="D" last="Letourneur">D. Letourneur</name><name sortKey="Viriot, M L" sort="Viriot, M L" uniqKey="Viriot M" first="M L" last="Viriot">M L Viriot</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Even, P" sort="Even, P" uniqKey="Even P" first="P" last="Even">P. Even</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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